Spine or Spinal may refer to:

• Spine (botany), needle-like structure
• Vertebral column, spine in anatomy, the backbone
• Dendritic spine, cellular neuroanatomy
• The spine of a book (see bookbinding)
• The Spine, a 2004 They Might Be Giants album, including the songs "Spine" and "Spines"
• Spinal (Killer Instinct), a fictional character from the video game Killer Instinct.