pBR322 is a plasmid and one of the most commonly used E. coli cloning vectors. pBR322 was the first artificial plasmid created by Boliviar and Rodriguez and was hence named eponymously after the scientist duo. pBR322 is 4363 base pairs in length and contains a replicon region (source plasmid pMB1); the tet gene, encodng the tetracycline resistance protein (source plasmid pSC101). It also contains the unique EcoRI restriction site GAATTC and splices the DNA between G and A from 5' to 3' position.

External links

• Overview
• PhD dissertation: Control of pBR322 plasmid replication in vivo, Chuen-Sheue Chiang, The University of Texas at Dallas
• Explanation of splice

PBR322