Mimivirus is a viral genus containing a single identified species named Acanthamoeba polyphaga mimivirus (APMV). In colloquial speech, APMV is more commonly referred to as just “mimivirus”. It has the largest capsid diameter of all known viruses, as well as a large and complex genome compared to other viruses. Though knowledge on the virus is relatively limited, the discovery of the virus excited many people due to the implications of its complex nature, with people hailing it as everything from a new domain of life to a missing link between viruses and bacteria.

Discovery

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APMV was discovered serendipitously in 1992 within the amoeba Acanthamoeba polyphaga, for which it is named, during research into Legionellosis. The virus was observed in a gram stain and mistakenly thought to be a gram-positive bacterium. As a consequence it was named "Bradfordcoccus", after the district the amoeba was sourced from in Bradford, England. In 2003, researchers at the Université de la Méditerranée in Marseille, France published a paper in Science identifying the micro-organism as a virus.

Mimivirus may be a causative agent of some forms of pneumonia, however, this is a tentative proposal based solely on indirect evidence in the form of antibodies to the virus discovered in pneumonia patients. Although it was once a suspect in the pneumonia outbreak in Bradford, today scientists believe that the virus can only infect amoebae.

Classification

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It has not been placed into a viral family by the International Committee on Taxonomy of Viruses but more members of the proposed family Mimiviridae are thought to exist based on metagenomic data.ICTV entry on Mimivirus. It has however, been placed into Group I of the Baltimore classification system.

Whilst not strictly a method of classification, Mimivirus joins a group of large viruses known as nucleocytoplasmic large DNA viruses (NCLDV), which includes four other families: Poxviridae, Iridoviridae, Phycodnaviridae and Asfarviridae. They are all large viruses which share both molecular characteristics and large genomes. The mimivirus genome also possesses 21 genes encoding homologs to proteins which are seen to be highly conserved in the majority of NCLDVs, and further work suggests that mimivirus is an early divergent of the general NCLDV groupLa Scola B, Audic S, Robert C, Jungang L, de Lamballerie X, Drancourt M, Birtles R, Claverie JM, Raoult D. A giant virus in amoebae. Science. 2003 Mar 28;299(5615):2033. PMID 12663918 .

Structure

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Mimivirus is the largest known virus, with a capsid diameter of 400 nm. Protein filaments measuring 100 nm project from the surface of the capsid, bringing the total length of the virus up to 600 nm. Variation in scientific literature renders these figures as highly approximate, with the “size” of the virion being casually listed as anywhere between 400 nm and 800 nm, depending on whether total length or capsid diameter is actually quoted. The capsid appears hexagonal under an electron microscope, therefore the capsid symmetry is icosahedral. It does not appear to possess an outer viral envelope, suggesting that the virus does not exit the host cell by exocytosisM. Suzan-Monti, B. La Scola and D. Raoult. Genomic and evolutionary aspects of Mimivirus. Virus Research, Volume 117, Issue 1, April 2006.

Mimivirus shares several morphological characteristics in common with all members of the NCLDV group of viruses. As an internal lipid layer surrounding the central core is present in all other NCLDV viruses, it has been suggested by M. Suzan-Monti et al. that this may also be present in mimivirus. The condensed central core of the virion appears as a dark region under the electron microscope. The large genome of the virus resides within this area.

Several mRNA transcripts can be recovered from purified virions. Like other NCLDVs, transcripts for DNA polymerase, a capsid protein and a TFII-like transcription factor were found. However, three distinct aminoacyl tRNA synthetase enzyme transcripts and four unknown mRNA molecules specific to mimivirus were also found. These pre-packaged transcripts can be translated without viral gene expression and are likely to be necessary to Mimivirus for replication. Other DNA viruses, such as the Human cytomegalovirus and Herpes simplex virus type-1, also feature pre-packaged mRNA transcripts (M. Suzan-Monti, 2006).

Genome

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The mimivirus genome is a linear, double-stranded molecule of DNA roughly 1.2 million base pairs in length. This makes it the largest viral genome in scientific knowledge, outstripping the next-largest virus genome of the myovirus Bacillus phage G by a little over double. In addition, it is larger than at least 30 cellular organismsJean-Michel Claveriea, Hiroyuki Ogataa, Stéphane Audica, Chantal Abergela, Karsten Suhrea and Pierre-Edouard Fourniera, Mimivirus and the emerging concept of “giant” virus. Virus Research, Volume 117, Issue 1, April 2006.

In addition to the large size of the genome, mimivirus possesses an estimated 911 protein-coding genes, far exceeding the minimum 4 genes required for viruses to exist (c.f. MS2 and Qβ virusesPrescott, L. (1993). Microbiology, Wm. C. Brown Publishers, ISBN 0697013723). Analysis of its genome revealed the presence of genes not seen in any other viruses, including aminoacyl tRNA synthetases, and other genes thought only to be encoded by cellular organisms. Like other large DNA viruses, mimivirus contains several genes for sugar, lipid and amino acid metabolism, as well as some metabolic genes not found in any other virus (M. Suzan-Monti, 2006). Roughly 90% of the genome was of coding capacity, with the other 10% being “junk DNA”.

Replication

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The stages of mimivirus replication are not well known, but as a minimum it is known that mimivirus attaches to a chemical receptor on the surface of an amoeba cell and is taken into the cell. Once inside, an eclipse phase begins, in which the virus disappears and all appears normal within the cell. After about four hours small accumulations can be seen in areas of the cell. Eight hours after infection many mimivirus virions are clearly visible within the cell. The cell cytoplasm continues to fill with newly synthesised virions and about 24 hours after initial infection the cell likely bursts open to release the new mimivirus virions (M. Suzan-Monti, 2006).

Little is known about the details of this replication cycle, most obviously attachment to the cell surface and entry, viral core release, DNA replication, transcription, translation, assembly and release of progeny virions. However, scientists have established the general overview given above using electron micrographs of infected cells. These micrographs show mimivirus capsid assembly in the nucleus, acquisition of an inner lipid membrane via budding from the nucleus, and particles similar to those found in many other viruses, including all NCLDV members. These particles are known in other viruses as viral factories and allow efficient viral assembly by modifying large areas the host cell.

Implications for life

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Mimivirus possesses many characteristics which place it at the boundary of living and non-living. It is as large as several bacterial species, namely Rickettsia conorii and Tropheryma whipplei, possesses a genome of comparable size to several bacteria, including those above, and codes for products previously not thought to be encoded by viruses. In addition, mimivirus possesses genes coding for nucleotide and amino acid synthesis, which even some small obligate intracellular bacteria lack. This means that unlike these bacteria, mimivirus is not dependant on the host cell genome for coding the metabolic pathways for these products. They do however, lack genes for ribosomal proteins, making mimivirus dependent for protein translation and energy metabolism. These factors combined have thrown scientists into debate over whether mimivirus is a distinct form of life, comparible on a domain scale to Eukaryota, Archaea and Prokaryota. However, it should be noted that mimivirus does not undergo cellular division, currently a basic requirement for life, instead replicating via self-assembly of individual components.

Because its lineage is very old and could have emerged prior to cellular organisms, mimivirus has added to the debate over the origins of life. Some genes unique to mimivirus, including those coding for the capsid, have been conserved in a variety of viruses which infect organisms from all domains - Eukaryota, Archaea and Prokaryota. This has been used to suggest that mimivirus emerged before cellular organisms and played a key role in the development of all of life on Earth Siebert, C., 2006, "Unintelligent Design," Discover 27 (3).

Other resources

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See also

• Mycoplasma genitalium - one of the smallest bacteria
• Pelagibacter ubique - possesses one of the smallest bacterial genomes
• Nanoarchaeum equitans - smallest known independant cell, smallest known genome
• Nanobacterium
• Nanobe
• Parvovirus - smallest known family of viruses

External links

The Big Picture Book of Viruses - images of mimivirus

Footnotes

References

Viruses

Mimivirus | Mimivirus | Mimivirus | Mimivirus | Mimivirus