Bovine serum albumin (BSA) can be used as a diluent or a blocking agent in numerous applications including ELISAs (Enzyme-Linked Immunosorbent Assay), blots and immunohistochemistry. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. This protein does not affect other enzymes that do not need it for stabilization. BSA is used because of its stability, its lack of effect in many biochemical reactions, and its low cost since it is readily available in large quatities as a byproduct of the beef industry.

BSA properties Amino acid: 582 Molecular weight: 66317.8 Theoretical pI: 5.65

References

• The sequence of BSA on NCBI.

runderalbumine