Penfield may refer to:

• Wilder Penfield (1891 – 1976), Canadian neurosurgeon
• The Prix Wilder-Penfield, an award for biomedicine research
• Daniel Penfield (1759 – 1840), founder of Penfield, New York
• Penfield, New York
• Penfield, Georgia
• Penfield Reef, a reef extending from Fairfield, Connecticut to the Long Island Sound
• The Penfield Reef Lighthouse
• The Louis Penfield House, a house built by Frank Lloyd Wright

Penfield may also refer to:

• Thomas Penfield Jackson (born 1937), former US District Court Judge