Mary Stuart may refer to:

• Mary I of Scotland, also known as "Mary, Queen of Scots", wife of King Francis II of France and mother of James I of England
• Mary Stuart (infant), daughter of James I of England who died in 1607 at age two
• Mary of Orange, Princess Royal and Princess of Orange-Nassau. Daughter of Charles I of England and mother of William III of England
• Mary II of England, co-ruler of England and Scotland with her husband William III from 1689 until her death in 1694
• Mary Stuart (actress), an American actress, most known for her 35-year role on Search for Tomorrow
• Maria Stuart (play), a tragedy by Friedrich Schiller