After Hours could refer to:

• After Hours, a popular Counter-Strike game server.
• After Hours, a movie directed by Martin Scorsese
• After Hours, an album by Gary Moore
• After hours trading of shares after the Stock Market has closed
• After Hours Formalwear, a business owned by May Department Stores
• "The After Hours", an episode of The Twilight Zone later remade for the 1980s revival of the series
• "After Hours", a song by Velvet Underground
• Afterhour clubs, underground rave-like clubs